A triply cloned strain of Xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange
Identifieur interne : 000213 ( France/Analysis ); précédent : 000212; suivant : 000214A triply cloned strain of Xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange
Auteurs : W. B. Li [Brésil] ; L. Zreik [France] ; N. G. Fernandes [Brésil] ; V. S. Miranda [Brésil] ; D. C. Teixeira [Brésil] ; A. J. Ayres [Brésil] ; M. Garnier [France] ; J. M. Bove [France]Source :
- Current microbiology [ 0343-8651 ] ; 1999.
Descripteurs français
- Pascal (Inist)
- Wicri :
- geographic : Brésil.
English descriptors
- KwdEn :
Abstract
Xylella fastidiosa isolate 8.1.b obtained from a sweet orange tree affected by citrus variegated chlorosis in the state of São Paulo, Brazil, and shown in 1993 to be the causal agent of the disease, was cloned by repeated culture in liquid and on solid PW medium, yielding triply cloned strain 9a5c. The eighth and the 16th passages of strain 9a5c were mechanically inoculated into sweet orange plants. Presence of X. fastidiosa in sweet orange leaves of shoots having grown after inoculation (first-flush shoots) was detected by DAS-ELISA and PCR. Thirty-eight days after inoculation, 70% of the 20 inoculated plants tested positive, and all plants gave strong positive reactions 90 days after inoculation. Symptoms first appeared after 3 months and were conspicuous after 5 months. X. fastidiosa was reisolated from sweet orange leaves, 44 days after inoculation. These results indicate that X. fastidiosa strain 9a5c, derived from pathogenic isolate 8.1.b by triply cloning, is also pathogenic. Strain 9a5c is now used for the X. fastidiosa genome sequencing project undertaken on a large scale in Brazil.
Affiliations:
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Pascal:99-0410858Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">A triply cloned strain of Xylella fastidiosa multiplies and induces symptoms of citrus variegated chlorosis in sweet orange</title>
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<sZ>5 aut.</sZ>
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<author><name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
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<country>Brésil</country>
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<author><name sortKey="Miranda, V S" sort="Miranda, V S" uniqKey="Miranda V" first="V. S." last="Miranda">V. S. Miranda</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Fundo Paulista de Defesa da Citricultura (Fundecitrus), Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, VI. Melhado</s1>
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<author><name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Fundo Paulista de Defesa da Citricultura (Fundecitrus), Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, VI. Melhado</s1>
<s2>Araraquara, São Paulo</s2>
<s3>BRA</s3>
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<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
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</author>
<author><name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Fundo Paulista de Defesa da Citricultura (Fundecitrus), Av. Dr. Adhemar Pereira de Barros, 201, 14807-040, VI. Melhado</s1>
<s2>Araraquara, São Paulo</s2>
<s3>BRA</s3>
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<country>Brésil</country>
<placeName><settlement type="city">São Paulo</settlement>
<region type="state">État de São Paulo</region>
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<author><name sortKey="Garnier, M" sort="Garnier, M" uniqKey="Garnier M" first="M." last="Garnier">M. Garnier</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux II, B.P. 81</s1>
<s2>33883 Villenave d'Ornon</s2>
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<sZ>8 aut.</sZ>
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<country>France</country>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Villenave d'Ornon</settlement>
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<author><name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J. M." last="Bove">J. M. Bove</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux II, B.P. 81</s1>
<s2>33883 Villenave d'Ornon</s2>
<s3>FRA</s3>
<sZ>2 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Villenave d'Ornon</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Current microbiology</title>
<title level="j" type="abbreviated">Cur. microbiol.</title>
<idno type="ISSN">0343-8651</idno>
<imprint><date when="1999">1999</date>
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<seriesStmt><title level="j" type="main">Current microbiology</title>
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<idno type="ISSN">0343-8651</idno>
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</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriosis</term>
<term>Brazil</term>
<term>Citrus sinensis</term>
<term>Inoculation</term>
<term>Isolate</term>
<term>Pathogenicity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Citrus sinensis</term>
<term>Pouvoir pathogène</term>
<term>Inoculation</term>
<term>Bactériose</term>
<term>Isolat</term>
<term>Brésil</term>
<term>Xylella fastidiosa</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Brésil</term>
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<front><div type="abstract" xml:lang="en">Xylella fastidiosa isolate 8.1.b obtained from a sweet orange tree affected by citrus variegated chlorosis in the state of São Paulo, Brazil, and shown in 1993 to be the causal agent of the disease, was cloned by repeated culture in liquid and on solid PW medium, yielding triply cloned strain 9a5c. The eighth and the 16th passages of strain 9a5c were mechanically inoculated into sweet orange plants. Presence of X. fastidiosa in sweet orange leaves of shoots having grown after inoculation (first-flush shoots) was detected by DAS-ELISA and PCR. Thirty-eight days after inoculation, 70% of the 20 inoculated plants tested positive, and all plants gave strong positive reactions 90 days after inoculation. Symptoms first appeared after 3 months and were conspicuous after 5 months. X. fastidiosa was reisolated from sweet orange leaves, 44 days after inoculation. These results indicate that X. fastidiosa strain 9a5c, derived from pathogenic isolate 8.1.b by triply cloning, is also pathogenic. Strain 9a5c is now used for the X. fastidiosa genome sequencing project undertaken on a large scale in Brazil.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
<li>France</li>
</country>
<region><li>Aquitaine</li>
<li>Nouvelle-Aquitaine</li>
<li>État de São Paulo</li>
</region>
<settlement><li>São Paulo</li>
<li>Villenave d'Ornon</li>
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<name sortKey="Ayres, A J" sort="Ayres, A J" uniqKey="Ayres A" first="A. J." last="Ayres">A. J. Ayres</name>
<name sortKey="Fernandes, N G" sort="Fernandes, N G" uniqKey="Fernandes N" first="N. G." last="Fernandes">N. G. Fernandes</name>
<name sortKey="Miranda, V S" sort="Miranda, V S" uniqKey="Miranda V" first="V. S." last="Miranda">V. S. Miranda</name>
<name sortKey="Teixeira, D C" sort="Teixeira, D C" uniqKey="Teixeira D" first="D. C." last="Teixeira">D. C. Teixeira</name>
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<country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Zreik, L" sort="Zreik, L" uniqKey="Zreik L" first="L." last="Zreik">L. Zreik</name>
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<name sortKey="Bove, J M" sort="Bove, J M" uniqKey="Bove J" first="J. M." last="Bove">J. M. Bove</name>
<name sortKey="Garnier, M" sort="Garnier, M" uniqKey="Garnier M" first="M." last="Garnier">M. Garnier</name>
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